protoscript ii first strand sequencing kit Search Results


protoscript ii first strand synthesis kit  (New England Biolabs)
97
New England Biolabs protoscript ii first strand synthesis kit
Protoscript Ii First Strand Synthesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c112 tianprep mini plasmid kit tiangen  (Vazyme Biotech Co)
99
Vazyme Biotech Co c112 tianprep mini plasmid kit tiangen
C112 Tianprep Mini Plasmid Kit Tiangen, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ultra ii rna sequencing kit  (New England Biolabs)
99
New England Biolabs ultra ii rna sequencing kit
Kraken2 classified read pair counts at the domain-level for (A) <t>RNA</t> shotgun library prepared samples, and (B) ToBRFV -Seq prepared samples . At the domain-level, categories included are viral, archaea, bacterial, eukaryotic, or other reads, where other includes unclassified reads, plasmids, adapters, linkers, and/or primers. (C) The percentage of reads represented by each domain for both prep methods. Samples A-E were used to calculate the average and the blank was excluded. (D&E) Percentage of Kraken2 classified ToBRFV (T), all other Tobamoviruses (OT), and the remainder of virus classified reads (RR) (F). Counts of Tobamovirus classified read pairs at the species-level (G). (A,B,D&E) Reads are represented on a log-scale to magnify and visualize low-abundance reads. Two-tailed tests were used to compare the mean read counts of RNA shotgun and ToBRFV -Seq classified reads, respectively. (D) RNA shotgun samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 3.569, (T-RR) −1.015, & (RR-OT) −1.181, critical t-value ±2.776. (E) ToBRFV -Seq samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 12.523, (T-RR) 12.523, & (RR-OT) 2.449, critical t-value ±2.776. Thus, there is a statistically significant difference between the number of ToBRFV and other Tobamovirus reads produced by RNA shotgun <t>sequencing.</t> Whereas, there is a statistically significant difference between the number of ToBRFV reads produced compared to both other Tobamovirus reads and the read remainder.
Ultra Ii Rna Sequencing Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protoscript ii first strand cdna synthesis kit  (New England Biolabs)
97
New England Biolabs protoscript ii first strand cdna synthesis kit
Kraken2 classified read pair counts at the domain-level for (A) <t>RNA</t> shotgun library prepared samples, and (B) ToBRFV -Seq prepared samples . At the domain-level, categories included are viral, archaea, bacterial, eukaryotic, or other reads, where other includes unclassified reads, plasmids, adapters, linkers, and/or primers. (C) The percentage of reads represented by each domain for both prep methods. Samples A-E were used to calculate the average and the blank was excluded. (D&E) Percentage of Kraken2 classified ToBRFV (T), all other Tobamoviruses (OT), and the remainder of virus classified reads (RR) (F). Counts of Tobamovirus classified read pairs at the species-level (G). (A,B,D&E) Reads are represented on a log-scale to magnify and visualize low-abundance reads. Two-tailed tests were used to compare the mean read counts of RNA shotgun and ToBRFV -Seq classified reads, respectively. (D) RNA shotgun samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 3.569, (T-RR) −1.015, & (RR-OT) −1.181, critical t-value ±2.776. (E) ToBRFV -Seq samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 12.523, (T-RR) 12.523, & (RR-OT) 2.449, critical t-value ±2.776. Thus, there is a statistically significant difference between the number of ToBRFV and other Tobamovirus reads produced by RNA shotgun <t>sequencing.</t> Whereas, there is a statistically significant difference between the number of ToBRFV reads produced compared to both other Tobamovirus reads and the read remainder.
Protoscript Ii First Strand Cdna Synthesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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d4200 dna clean concentrator 5 zymo research  (Zymo Research)
99
Zymo Research d4200 dna clean concentrator 5 zymo research
Kraken2 classified read pair counts at the domain-level for (A) <t>RNA</t> shotgun library prepared samples, and (B) ToBRFV -Seq prepared samples . At the domain-level, categories included are viral, archaea, bacterial, eukaryotic, or other reads, where other includes unclassified reads, plasmids, adapters, linkers, and/or primers. (C) The percentage of reads represented by each domain for both prep methods. Samples A-E were used to calculate the average and the blank was excluded. (D&E) Percentage of Kraken2 classified ToBRFV (T), all other Tobamoviruses (OT), and the remainder of virus classified reads (RR) (F). Counts of Tobamovirus classified read pairs at the species-level (G). (A,B,D&E) Reads are represented on a log-scale to magnify and visualize low-abundance reads. Two-tailed tests were used to compare the mean read counts of RNA shotgun and ToBRFV -Seq classified reads, respectively. (D) RNA shotgun samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 3.569, (T-RR) −1.015, & (RR-OT) −1.181, critical t-value ±2.776. (E) ToBRFV -Seq samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 12.523, (T-RR) 12.523, & (RR-OT) 2.449, critical t-value ±2.776. Thus, there is a statistically significant difference between the number of ToBRFV and other Tobamovirus reads produced by RNA shotgun <t>sequencing.</t> Whereas, there is a statistically significant difference between the number of ToBRFV reads produced compared to both other Tobamovirus reads and the read remainder.
D4200 Dna Clean Concentrator 5 Zymo Research, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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assays plasmacytoid dendritic cell isolation kit ii  (Miltenyi Biotec)
95
Miltenyi Biotec assays plasmacytoid dendritic cell isolation kit ii
Kraken2 classified read pair counts at the domain-level for (A) <t>RNA</t> shotgun library prepared samples, and (B) ToBRFV -Seq prepared samples . At the domain-level, categories included are viral, archaea, bacterial, eukaryotic, or other reads, where other includes unclassified reads, plasmids, adapters, linkers, and/or primers. (C) The percentage of reads represented by each domain for both prep methods. Samples A-E were used to calculate the average and the blank was excluded. (D&E) Percentage of Kraken2 classified ToBRFV (T), all other Tobamoviruses (OT), and the remainder of virus classified reads (RR) (F). Counts of Tobamovirus classified read pairs at the species-level (G). (A,B,D&E) Reads are represented on a log-scale to magnify and visualize low-abundance reads. Two-tailed tests were used to compare the mean read counts of RNA shotgun and ToBRFV -Seq classified reads, respectively. (D) RNA shotgun samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 3.569, (T-RR) −1.015, & (RR-OT) −1.181, critical t-value ±2.776. (E) ToBRFV -Seq samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 12.523, (T-RR) 12.523, & (RR-OT) 2.449, critical t-value ±2.776. Thus, there is a statistically significant difference between the number of ToBRFV and other Tobamovirus reads produced by RNA shotgun <t>sequencing.</t> Whereas, there is a statistically significant difference between the number of ToBRFV reads produced compared to both other Tobamovirus reads and the read remainder.
Assays Plasmacytoid Dendritic Cell Isolation Kit Ii, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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assays nebnext ultra iitm dna library prep kit new england biolabs  (New England Biolabs)
95
New England Biolabs assays nebnext ultra iitm dna library prep kit new england biolabs
Kraken2 classified read pair counts at the domain-level for (A) <t>RNA</t> shotgun library prepared samples, and (B) ToBRFV -Seq prepared samples . At the domain-level, categories included are viral, archaea, bacterial, eukaryotic, or other reads, where other includes unclassified reads, plasmids, adapters, linkers, and/or primers. (C) The percentage of reads represented by each domain for both prep methods. Samples A-E were used to calculate the average and the blank was excluded. (D&E) Percentage of Kraken2 classified ToBRFV (T), all other Tobamoviruses (OT), and the remainder of virus classified reads (RR) (F). Counts of Tobamovirus classified read pairs at the species-level (G). (A,B,D&E) Reads are represented on a log-scale to magnify and visualize low-abundance reads. Two-tailed tests were used to compare the mean read counts of RNA shotgun and ToBRFV -Seq classified reads, respectively. (D) RNA shotgun samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 3.569, (T-RR) −1.015, & (RR-OT) −1.181, critical t-value ±2.776. (E) ToBRFV -Seq samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 12.523, (T-RR) 12.523, & (RR-OT) 2.449, critical t-value ±2.776. Thus, there is a statistically significant difference between the number of ToBRFV and other Tobamovirus reads produced by RNA shotgun <t>sequencing.</t> Whereas, there is a statistically significant difference between the number of ToBRFV reads produced compared to both other Tobamovirus reads and the read remainder.
Assays Nebnext Ultra Iitm Dna Library Prep Kit New England Biolabs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hieff ngs maxup ii dna library prep kit illumina  (Yeasen Biotechnology)
90
Yeasen Biotechnology hieff ngs maxup ii dna library prep kit illumina
Kraken2 classified read pair counts at the domain-level for (A) <t>RNA</t> shotgun library prepared samples, and (B) ToBRFV -Seq prepared samples . At the domain-level, categories included are viral, archaea, bacterial, eukaryotic, or other reads, where other includes unclassified reads, plasmids, adapters, linkers, and/or primers. (C) The percentage of reads represented by each domain for both prep methods. Samples A-E were used to calculate the average and the blank was excluded. (D&E) Percentage of Kraken2 classified ToBRFV (T), all other Tobamoviruses (OT), and the remainder of virus classified reads (RR) (F). Counts of Tobamovirus classified read pairs at the species-level (G). (A,B,D&E) Reads are represented on a log-scale to magnify and visualize low-abundance reads. Two-tailed tests were used to compare the mean read counts of RNA shotgun and ToBRFV -Seq classified reads, respectively. (D) RNA shotgun samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 3.569, (T-RR) −1.015, & (RR-OT) −1.181, critical t-value ±2.776. (E) ToBRFV -Seq samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 12.523, (T-RR) 12.523, & (RR-OT) 2.449, critical t-value ±2.776. Thus, there is a statistically significant difference between the number of ToBRFV and other Tobamovirus reads produced by RNA shotgun <t>sequencing.</t> Whereas, there is a statistically significant difference between the number of ToBRFV reads produced compared to both other Tobamovirus reads and the read remainder.
Hieff Ngs Maxup Ii Dna Library Prep Kit Illumina, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sequel ii sequencing kit 2.0  (Pacific Biosciences)
90
Pacific Biosciences sequel ii sequencing kit 2.0
Kraken2 classified read pair counts at the domain-level for (A) <t>RNA</t> shotgun library prepared samples, and (B) ToBRFV -Seq prepared samples . At the domain-level, categories included are viral, archaea, bacterial, eukaryotic, or other reads, where other includes unclassified reads, plasmids, adapters, linkers, and/or primers. (C) The percentage of reads represented by each domain for both prep methods. Samples A-E were used to calculate the average and the blank was excluded. (D&E) Percentage of Kraken2 classified ToBRFV (T), all other Tobamoviruses (OT), and the remainder of virus classified reads (RR) (F). Counts of Tobamovirus classified read pairs at the species-level (G). (A,B,D&E) Reads are represented on a log-scale to magnify and visualize low-abundance reads. Two-tailed tests were used to compare the mean read counts of RNA shotgun and ToBRFV -Seq classified reads, respectively. (D) RNA shotgun samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 3.569, (T-RR) −1.015, & (RR-OT) −1.181, critical t-value ±2.776. (E) ToBRFV -Seq samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 12.523, (T-RR) 12.523, & (RR-OT) 2.449, critical t-value ±2.776. Thus, there is a statistically significant difference between the number of ToBRFV and other Tobamovirus reads produced by RNA shotgun <t>sequencing.</t> Whereas, there is a statistically significant difference between the number of ToBRFV reads produced compared to both other Tobamovirus reads and the read remainder.
Sequel Ii Sequencing Kit 2.0, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid mini kit ii  (Omega Bio Tek)
95
Omega Bio Tek plasmid mini kit ii
Kraken2 classified read pair counts at the domain-level for (A) <t>RNA</t> shotgun library prepared samples, and (B) ToBRFV -Seq prepared samples . At the domain-level, categories included are viral, archaea, bacterial, eukaryotic, or other reads, where other includes unclassified reads, plasmids, adapters, linkers, and/or primers. (C) The percentage of reads represented by each domain for both prep methods. Samples A-E were used to calculate the average and the blank was excluded. (D&E) Percentage of Kraken2 classified ToBRFV (T), all other Tobamoviruses (OT), and the remainder of virus classified reads (RR) (F). Counts of Tobamovirus classified read pairs at the species-level (G). (A,B,D&E) Reads are represented on a log-scale to magnify and visualize low-abundance reads. Two-tailed tests were used to compare the mean read counts of RNA shotgun and ToBRFV -Seq classified reads, respectively. (D) RNA shotgun samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 3.569, (T-RR) −1.015, & (RR-OT) −1.181, critical t-value ±2.776. (E) ToBRFV -Seq samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 12.523, (T-RR) 12.523, & (RR-OT) 2.449, critical t-value ±2.776. Thus, there is a statistically significant difference between the number of ToBRFV and other Tobamovirus reads produced by RNA shotgun <t>sequencing.</t> Whereas, there is a statistically significant difference between the number of ToBRFV reads produced compared to both other Tobamovirus reads and the read remainder.
Plasmid Mini Kit Ii, supplied by Omega Bio Tek, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti phospho pkc isoform sampler kit  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc anti phospho pkc isoform sampler kit
Kraken2 classified read pair counts at the domain-level for (A) <t>RNA</t> shotgun library prepared samples, and (B) ToBRFV -Seq prepared samples . At the domain-level, categories included are viral, archaea, bacterial, eukaryotic, or other reads, where other includes unclassified reads, plasmids, adapters, linkers, and/or primers. (C) The percentage of reads represented by each domain for both prep methods. Samples A-E were used to calculate the average and the blank was excluded. (D&E) Percentage of Kraken2 classified ToBRFV (T), all other Tobamoviruses (OT), and the remainder of virus classified reads (RR) (F). Counts of Tobamovirus classified read pairs at the species-level (G). (A,B,D&E) Reads are represented on a log-scale to magnify and visualize low-abundance reads. Two-tailed tests were used to compare the mean read counts of RNA shotgun and ToBRFV -Seq classified reads, respectively. (D) RNA shotgun samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 3.569, (T-RR) −1.015, & (RR-OT) −1.181, critical t-value ±2.776. (E) ToBRFV -Seq samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 12.523, (T-RR) 12.523, & (RR-OT) 2.449, critical t-value ±2.776. Thus, there is a statistically significant difference between the number of ToBRFV and other Tobamovirus reads produced by RNA shotgun <t>sequencing.</t> Whereas, there is a statistically significant difference between the number of ToBRFV reads produced compared to both other Tobamovirus reads and the read remainder.
Anti Phospho Pkc Isoform Sampler Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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zymoprep yeast plasmid miniprep ii kit  (Zymo Research)
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Zymo Research zymoprep yeast plasmid miniprep ii kit
KEY RESOURCES TABLE
Zymoprep Yeast Plasmid Miniprep Ii Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Kraken2 classified read pair counts at the domain-level for (A) RNA shotgun library prepared samples, and (B) ToBRFV -Seq prepared samples . At the domain-level, categories included are viral, archaea, bacterial, eukaryotic, or other reads, where other includes unclassified reads, plasmids, adapters, linkers, and/or primers. (C) The percentage of reads represented by each domain for both prep methods. Samples A-E were used to calculate the average and the blank was excluded. (D&E) Percentage of Kraken2 classified ToBRFV (T), all other Tobamoviruses (OT), and the remainder of virus classified reads (RR) (F). Counts of Tobamovirus classified read pairs at the species-level (G). (A,B,D&E) Reads are represented on a log-scale to magnify and visualize low-abundance reads. Two-tailed tests were used to compare the mean read counts of RNA shotgun and ToBRFV -Seq classified reads, respectively. (D) RNA shotgun samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 3.569, (T-RR) −1.015, & (RR-OT) −1.181, critical t-value ±2.776. (E) ToBRFV -Seq samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 12.523, (T-RR) 12.523, & (RR-OT) 2.449, critical t-value ±2.776. Thus, there is a statistically significant difference between the number of ToBRFV and other Tobamovirus reads produced by RNA shotgun sequencing. Whereas, there is a statistically significant difference between the number of ToBRFV reads produced compared to both other Tobamovirus reads and the read remainder.

Journal: bioRxiv

Article Title: A Novel Tiled-Amplicon Sequencing Assay Targeting the Tomato Brown Rugose Fruit Virus ( ToBRFV ) Genome Reveals Widespread Distribution in Municipal Wastewater Treatment Systems in the Province of Ontario, Canada

doi: 10.1101/2023.11.02.565359

Figure Lengend Snippet: Kraken2 classified read pair counts at the domain-level for (A) RNA shotgun library prepared samples, and (B) ToBRFV -Seq prepared samples . At the domain-level, categories included are viral, archaea, bacterial, eukaryotic, or other reads, where other includes unclassified reads, plasmids, adapters, linkers, and/or primers. (C) The percentage of reads represented by each domain for both prep methods. Samples A-E were used to calculate the average and the blank was excluded. (D&E) Percentage of Kraken2 classified ToBRFV (T), all other Tobamoviruses (OT), and the remainder of virus classified reads (RR) (F). Counts of Tobamovirus classified read pairs at the species-level (G). (A,B,D&E) Reads are represented on a log-scale to magnify and visualize low-abundance reads. Two-tailed tests were used to compare the mean read counts of RNA shotgun and ToBRFV -Seq classified reads, respectively. (D) RNA shotgun samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 3.569, (T-RR) −1.015, & (RR-OT) −1.181, critical t-value ±2.776. (E) ToBRFV -Seq samples, p < 0.05, n = 5, df = 4, t-values, (T-OT) 12.523, (T-RR) 12.523, & (RR-OT) 2.449, critical t-value ±2.776. Thus, there is a statistically significant difference between the number of ToBRFV and other Tobamovirus reads produced by RNA shotgun sequencing. Whereas, there is a statistically significant difference between the number of ToBRFV reads produced compared to both other Tobamovirus reads and the read remainder.

Article Snippet: RNA extracts from WWI were prepared for RNA sequencing using the NEB Ultra II RNA Sequencing kit ( New England Biolabs , E7770S), following manufacturer’s recommended protocol, section 5.

Techniques: Virus, Two Tailed Test, Produced, Shotgun Sequencing

KEY RESOURCES TABLE

Journal: Cell

Article Title: Decoupling the Functional Pleiotropy of Stem Cell Factor by Tuning c-Kit Signaling

doi: 10.1016/j.cell.2017.02.011

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Zymoprep ™ Yeast Plasmid Miniprep II Kit , Zymo Research , Cat#D2004.

Techniques: Recombinant, Mutagenesis, Electron Microscopy, Staining, Modification, Giemsa Stain, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Endotoxin Assay, Selection, Expressing, Sequencing, Software, Diagnostic Assay, Live Cell Imaging